Next Generation Genome Sequencing

The Genetic Sequencing Facility (GSC) is one of three research core labs in the newly established NIH COBRE Center for Molecular Analysis of Disease Pathways (CMADP) at KU. The major mission of the GSC is to provide researchers with next-generation sequencing (NGS) technologies as well as expertise in experimental design and analysis of sequence data. Projects supported by the GSC include de novo genome assembly, genome re-sequencing for identification of mutations and polymorphisms, transcriptome analysis (RNA-Seq), epigenomic and gene regulation studies such as ChIP-Seq, Methyl-Seq, small RNA discovery and analysis. The Illumina HiSeq 2500 next-generation sequencer has the capacity to generate 3-6 billion reads of 100bp per run of two eight-lane flow cells (600Gb data). In its rapid mode, it can generate 1.2 billion reads of 150 bp per run on two two-lane flow cells (120Gb data) in 27 hours. To capture the full power of NGS, the core provides a whole range of project support, from project consultation, sample QC, library construction, cluster generation, to data generation.

More details
  • Provides education, lab training, and assistance to researchers in experimental design, data handling, and new method development
  • High output (more data, longer wait time) and rapid run (quicker turnaround, less data) available
  • Library preparation
Jennifer Hackett, M.S.
Haworth Hall, room 1030

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