| Access to zebrafish embryos, larvae, and adults for research |
Molecular Probes |
| Multicolor flow cytometry |
Immunology |
| ELISA |
Immunology |
| Cell based assays |
Immunology |
| Aseptic cell sorting |
Immunology |
| Fluorescence activated cell sorting |
Immunology |
| Cytokine quantification |
Immunology |
| ELISpot |
Immunology |
| Synthesis of fluorescent molecular probes (fluorophores, small molecules, peptides) |
Molecular Probes |
| Fluorescence and brightfield imaging of zebrafish |
Molecular Probes |
| Fluorescence dissecting microscopy |
Molecular Probes |
| Pharmacology/toxicology screening of chemical libraries against zebrafish embryos |
Molecular Probes |
| Assay development, fluorescence-based using mammalian cells, zebrafish and C. elegans |
Molecular Probes |
| Generation of zebrafish embryos for phenotypic screening and discovery toxicology |
Molecular Probes |
| Mammalian cell culture |
Molecular Probes |
| Microinjection of model organisms |
Molecular Probes |
| Protein digestion |
Analytical Proteomics |
| Laboratory automation |
Instrumentation Design |
| Label free protein interaction studies |
Protein Production Group |
| Biacore label-free binding studies |
Protein Production Group |
| Fragment library screening by SPR |
Protein Production Group |
| Microarray data generation and analysis |
Genomics Facility |
| Real-time PCR |
Genomics Facility |
| Target identification and localization using affinity labeling of probe compounds |
Specialized Chemistry Center |
| Multispectral in vivo imaging |
Experimental and Preclinical Imaging |
| Induction of DNA strand breaks |
Biological Irradiation |
| Machine learning and data mining for large scale, multidimensional chemical biology data sets |
Molecular Graphics and Modeling |
| Docking and virtual screening of small molecule ligands against protein targets of interest |
Molecular Graphics and Modeling |
| Molecular dynamics simulation and modeling of small/macro molecules, proteins, andbiomolecular systems |
Molecular Graphics and Modeling |
| Analysis of structure-activity relationships |
Molecular Graphics and Modeling |
| Visualization and graphics of biomolecules and biomolecular systems |
Molecular Graphics and Modeling |
| Cell survival studies |
Biological Irradiation |
| Radiation treatment studies |
Biological Irradiation |
| Bone density measurements |
Experimental and Preclinical Imaging |
| Preclincial drug efficacy |
Experimental and Preclinical Imaging |
| Identification of protein targets of probe compounds using yeast three-hybrid systems |
Specialized Chemistry Center |
| Protein structure solution refinement, model building and analysis |
Protein Structure Lab |
| Protein crystallization |
Protein Structure Lab |
| X-ray diffraction data collection (in-house) |
Protein Production Group |
| Synchrotron X-ray diffraction data collection |
Protein Production Group |
| Protein characterization and analysis |
Protein Production Group |
| Compound identity and purity verification |
Medicinal Chemistry |
| Compound reaction sequences (multi-step) |
Medicinal Chemistry |
| Compound property predictions |
Medicinal Chemistry |
| Protein structure, function and stability prediction, sequence and domain analyses |
Applied Bioinformatics |
| Data analysis and mining in proteomics, genomics and chemistry |
Applied Bioinformatics |
| Phylogenetic analysis |
Applied Bioinformatics |
| Fragment library screening by STD-NMR |
Biomolecular NMR |
| NMR data processing, analysis and interpretation |
Biomolecular NMR |
| 2D-1H-15-HSQC, 2D-1H-13C-HSQC |
Biomolecular NMR |
| Structure determination of peptides, proteins, nucleic acids and its complexes |
Biomolecular NMR |
| Protein-ligand (drug, peptide, protein) detailed interaction study |
Biomolecular NMR |
| Absolute configurations for small molecules |
X-ray Crystallography |
| Solid-state structure determinations for microcrystals |
X-ray Crystallography |
| Variable-temperature small-molecule and macromolecular/protein diffraction data collection, analysis and interpretation |
X-ray Crystallography |
| Phase identification and characterization for crystalline materials |
X-ray Crystallography |
| Protein-ligand (drug, peptide, protein) detailed interaction studies in solid-state |
X-ray Crystallography |
| Quantitative Powder X-Ray Diffraction (PXRD) data collection and interpretation |
X-ray Crystallography |
| Rapid qualitative Powder X-Ray Diffraction (PXRD) data collection and interpretation for microgram samples |
X-ray Crystallography |
| Small molecule crystallization |
X-ray Crystallography |
| Small molecule (Cambridge) crystal structure database search |
X-ray Crystallography |
| Solid-state structure determinations for small organic and inorganic molecules and biological molecules |
X-ray Crystallography |
| Thin-film characterization |
X-ray Crystallography |
| Protein structure determination |
Protein Structure |
| Protein molecular weight determination |
Analytical Proteomics |
| Post-translational modification determinations of proteins (i.e. phosphorylation, acetylation) |
Analytical Proteomics |
| MALDI-TOF MS analysis of proteins and peptides |
Analytical Proteomics |
| LC-MS analysis of peptides |
Analytical Proteomics |
| Protein sequence database search |
Analytical Proteomics |
| Protein identification |
Mass Spectrometry |
| Physicochemical profiling |
BIO Center |
| Scanning electron microscopy |
Microscopy and Analytical Imaging |
| Light Microscopy, epifluorescence, confocal, and TIRF |
Microscopy and Analytical Imaging |
| Live cell imaging |
Microscopy and Analytical Imaging |
| Fluorescence activated cell sorting |
Microscopy and Analytical Imaging |
| Sample prep for light and electron microscopy |
Microscopy and Analytical Imaging |
| Scanning probe microscopy |
Microscopy and Analytical Imaging |
| Assay development, analysis and data exploration |
Microscopy and Analytical Imaging |
| Transmission electron microscopy |
Microscopy and Analytical Imaging |
| Bioavailability and Pharmacokinetics |
BIO Center |
| Bio-molecule assays |
BIO Center |
| Cell-based assays |
BIO Center |
| Prodrug design and synthesis |
BIO Center |
| Peptide and small molecule synthesis |
BIO Center |
| Hepatic microsomal stability assesment |
BIO Center |
| Protein binding and plasma stability |
BIO Center |
| Pharmacokinetic (PK) screening |
BIO Center |
| Small molecule structure elucidation |
Nuclear Magnetic Resonance |
| 1D 1H NMR |
Nuclear Magnetic Resonance |
| 1D 13C NMR |
Nuclear Magnetic Resonance |
| Hetronuclear NMR (15N, 19F, 31P, etc.) |
Nuclear Magnetic Resonance |
| Quantitative NMR |
Nuclear Magnetic Resonance |
| Variable temperature NMR |
Nuclear Magnetic Resonance |
| Material characterization in solid state |
Solid State NMR |
| Degree of crosslinking in polymers |
Solid State NMR |
| HPLC or UPLC/MS or HPLC MS/MS |
Mass Spectrometry |
| Electrospray ionization (ESI-MS) |
Mass Spectrometry |
| Whole genome sequencing |
Genome Sequencing |
| ChIP-seq |
Genome Sequencing |
| Methylated DNA sequencing |
Genome Sequencing |
| RNA-seq |
Genome Sequencing |
| DNA next generation sequencing |
Genome Sequencing |
| Genome de novo sequencing or re-sequencing |
Genome Sequencing |
| Physical properties and transport of biological fluids |
Microfabrication and Microfluidics |
| RNA-seq data analysis |
Genomics and Bioinformatics |
| ChIP-seq data analysis |
Genomics and Bioinformatics |
| RAD (restriction-site associated DNA) sequencing analysis |
Genomics and Bioinformatics |
| Whole genome assembly |
Genomics and Bioinformatics |
| Bioanalytical studies (small and large molecules) |
Microfabrication and Microfluidics |
| Cell-based assays |
High Throughput Screening |
| Enzyme/protein assays |
High Throughput Screening |
| Compound screening and profiling |
High Throughput Screening |
| Compound synthesis |
Chemical Methodologies and Library Dev. |
| Compound library design |
Chemical Methodologies and Library Dev. |
